Helicobacter pylori promotes gastric cancer metastasis via up-regulating the expression of Bmi-1 / 细胞与分子免疫学杂志

Objective To clarify whether Helicobacter pylori (H. pylori) can promote metastasis of gastric cancer cells via the high-expression of induced B cell specific Moloney murine leukemia virus integration site 1 (Bmi-1). Methods The gastric cancer tissue specimens from 82 patients were collected for this study. The protein and gene expression level of Bmi-1 in gastric adenocarcinoma tissue were detected by immunohistochemistry and real time quantitative PCR, respectively. And meanwhile the correlation between Bmi-1 levels and pathological features, and prognosis of gastric cancer were analyzed retrospectively. Then, the GES-1 cells were transfected with pLPCX-Bmi-1 plasmid and infected with H. pylori respectively. After the Bmi-1 overexpression in GES-1 cells, the invasion ability of the GES-1 cells was detected by Transwell assay, and the cell cycle and apoptosis were detected by flow cytometry. Results The mRNA and protein of Bmi-1 expression in gastric cancer tissues were higher than tumor-adjacent tissue, and the high expression of Bmi-1 was positively correlated with tumor invasion, TNM stage, tumor differentiation, lymph node metastasis and H. pylori infection. When expression of Bmi-1 was up-regulated as a result of H.pylori infection or pLPCX-Bmi-1 transfection, the GES-1 cells had higher invasiveness and lower apoptosis rate with the above treatment respectively. Conclusion H. pylori infection can inhibit the apoptosis of gastric cancer cells and promote their invasion via up-regulating expression of Bmi-1.

Timing of Indomethacin suppositories for the prevention of post-ERCP pancreatitis in high risk groups / 中国内镜杂志

Objective A prospective randomized controlled trial was carried out to explore the best time of In-domethacin suppositories administration for the prevention of post-ERCP pancreatitis in high-risk groups. Methods 81 patients were enrolled in the study finally. Patients were randomized into group A (100 mg rectal Indomethacin suppositories was administrated immediately after ERCP), group B (100 mg rectal Indomethacin suppositories was administrated half an hour after ERCP) and group C (ERCP alone group, which did not give Indomethacin supposito-ries). The level of serum amylase, urine amylase, serum CRP, serum IL-6, serum TNF-α were measured before, 3 h, 24 h and 48 h after ERCP, and the incidence of PEP and hyperamylasemia were analyzed. Results There was 1 case (4.00%) of PEP in group A, 2 cases (5.41%) of PEP in group B and 5 cases (26.31%) of PEP in group C;the incidences of PEP of group A and group B were significant lower than that in group C (P < 0.05). There was 1 case (4.00%) of hyperamylasemia in group A, 5 cases (13.51 %) of hyperamylasemia in group B and 6 cases (31.6 %) of hyperamylasemia in group C, and the incidences of hyperamylasemia of group A and group B were significant lower than that in group C ( P< 0.05). Conclusion Administration of 100 mg Indomethacin suppositories immediately or half an hour after ERCP can effectively reduce the incidence of PEP and hyperamylasemia.

Clinical Observation on Effect of Flupentixol and Melitracen Combined with Quadruple Therapy on Peptic Ulcer Patients with Anxiety and Depression / 胃肠病学

Background:Studies have shown that patients with peptic ulcer are susceptible to the accompanying of anxiety and depression,and affects the treatment of ulcer. Aims:To study the effect of flupentixol and melitracen combined with quadruple therapy on peptic ulcer patients with anxiety and depression. Methods:Sixty-eight peptic ulcer patients with anxiety and depression from May 2012 to May 2013 at Wuhu No. 2 People’s Hospital were enrolled,and were randomly divided into control group and observation group. Patients in control group were given quadruple therapy( rabeprazole 20 mg + amoxicillin 1. 0 g + clarithromycin 0. 5 g + colloidal bismuth subcitrate solution 10 mL,bid),while patients in observation group were given flupentixol and melitracen in addition to quadruple therapy,the treatment course were both for 2 weeks. Therapeutic efficacy of ulcer,Helicobacter pylori( Hp) eradication,therapeutic efficacy of anxiety and depression,incidence of adverse reaction and recurrence rate of ulcer within one year after treatment were compared between the two groups. Results:Compared with control group,the therapeutic efficacy of ulcer and Hp eradication in observation group were significantly increased(P < 0. 05),therapeutic efficacy of anxiety and depression was significantly increased(P < 0. 05),no significant difference in the incidence of adverse reaction was found between the two groups,and the recurrence rate of ulcer within one year was significantly decreased( P < 0. 05). Conclusions:Flupentixol and melitracen combined with quadruple therapy could significantly improve the therapeutic efficacy and effectively reduce the recurrence rate in peptic ulcer patients with anxiety and depression.

Preparation of hepatoblasts from fetal liver tissues / 中国组织工程研究

BACKGROUND:Hepatoblasts are one of high-grade cell sources for severe liver disease treatment.Efficient separation and purification of hepatoblasts from fetal liver has important practical significances.OBJECTIVE:To introduce the preparation of hepatoblasts from fetal liver,conduct a transversal comparison of these preparation methods,and point out their advantages and shortcomings,so as to provide the evidences for the experimental and clinical choices of hepatoblasts.RETRIEVAL STRATEGY:Using the keywords of "fetal liver progenitor/hepatoblasts,isolation/purification",we searched the articles about fetal liver hepatoblasts separation and purification in PUBMED between January 1997 and August 2007 in English.At the same time,we searched the relative articles in CNKI using the keywords of "liver,embryo,endothelial progenitor/hepatoblasts" between January 1997 and August 2007 in Chinese.In addition,some related books and foreign articles were checked in the laboratory of Jilin University.After the first examination,the articles published in the authority magazines within five years were given prior consideration.Exclusive criteria were those repetitive researches or Meta-analysis.Totally 245 articles were collected,we selected 33 representative articles about hepatoblasts separation and purification methods.LITERATURE EVALUATION:Among 31 included articles,10 ones introduced the study background,5 ones studied the fetal liver cell dissociation,9 ones referred to the hepatoblasts separation and purification,and 7 ones indicated the problems and prospect in this field.DATA SYNTHESIS:The hepatic epithelial cell precursor cells(also known as hepatoblasts) with hepatic and biliary epithelial cell differentiation potential isolated from fetal liver,could in vitro proliferate and differentiate,after transplantation in vivo they show a good homing,and the ability to integrate and regenerate.Hepatoblasts isolation technology is being improved daily,and the related researches on this field will boost cell-based liver therapy in clinical application.CONCLUSION:Improvements and combination of the various isolation methods,as well as new and high-specific antigen discovery will further improve the isolation efficiency of hepatoblasts from fetal liver.